Human Skin Collagenase: Localization and Distribution in Normal Human Skin*
نویسندگان
چکیده
منابع مشابه
Purification, characterization and inhibition of human skin collagenase.
1. The neutral collagenase released into the culture medium by explants of human skin tissue was purified by ultrafiltration and column chromatography. The final enzyme preparation had a specific activity against thermally reconstituted collagen fibrils of 32mug of collagen degraded/min per mg of enzyme protein, representing a 266-fold increase over that of the culture medium. Electrophoresis i...
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The biosynthesis of human skin collagenase was studied in fibroblast cultures incubated with 13H]leutine under serum-free conditions. The radioactively labeled enzyme was isolated and quantitated directly from culture medium using a highly specific immunoprecipitation method. Sodium dodecyl sulfate gel electrophoresis of the immunoprecipitates showed two radioactive bands tihich co-migrated wit...
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The gelatinolytic activity of human skin fibroblast collagenase was examined on denatured collagen types I-V. All denatured substrates were cleaved, including types IV and V, which are resistant to collagenase in native form. Interestingly, the earliest major cleavage in denatured collagen types I-III occurred at a 3/4-1/4 locus, resulting in products electrophoretically identical with TCA and ...
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In order to gain insight into the biological significance of a collagenase inhibitor secreted by human skin fibroblasts, we examined various human connective tissues and body fluids for such a protein. The inhibitors found in these tissues were compared immunologically to skin fibroblast inhibitor by Ouchterlony analysis and by the development of a highly specific enzyme-linked immunosorbent as...
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Background: Sulfur mustard gas is a chemical agent that has been used in many wars, especially in Iran-Iraq war. This chemical agent affects many organs including lungs, eyes and skin, causing numerous acute and chronic lesions including erythema and hyperpigmentation, respectively. This study was conducted to evaluate erythema and melanin in subjects with a history of exposure to sulfur ...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1969
ISSN: 0022-202X
DOI: 10.1038/jid.1969.76